colony-stimulating factor Search Results


recombinant mcsf  (Gold Biotechnology Inc)
93
Gold Biotechnology Inc recombinant mcsf
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Recombinant Mcsf, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hy p7085  (MedChemExpress)
96
MedChemExpress hy p7085
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Hy P7085, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 5  (Boster Bio)
93
Boster Bio il 5
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Il 5, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p acnes camp factor  (ProSci Incorporated)
90
ProSci Incorporated p acnes camp factor
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
P Acnes Camp Factor, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csf antibody  (Proteintech)
93
Proteintech csf antibody
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Csf Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macrophage colony  (MedChemExpress)
94
MedChemExpress macrophage colony
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 3  (MedChemExpress)
93
MedChemExpress il 3
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Il 3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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granuocyte macrophage colony  (MedChemExpress)
94
MedChemExpress granuocyte macrophage colony
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Granuocyte Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human g csf  (Boster Bio)
93
Boster Bio human g csf
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Human G Csf, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csf1r inhibitors  (MedChemExpress)
93
MedChemExpress csf1r inhibitors
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Csf1r Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igf 1  (MedChemExpress)
94
MedChemExpress igf 1
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Igf 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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granulocyte colony stimulating factor g csf  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc granulocyte colony stimulating factor g csf
Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated <t>stimuli:</t> <t>GMCSF</t> (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: <t>MCSF</t> (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD
Granulocyte Colony Stimulating Factor G Csf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated stimuli: GMCSF (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: MCSF (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD

Journal: BMC Immunology

Article Title: Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients

doi: 10.1186/s12865-021-00410-2

Figure Lengend Snippet: Cell surface differentiation marker characterization of polarized macrophages. M1 macrophages were polarized using indicated stimuli: GMCSF (20 ng/ml) for 5 days followed by LPS (10 ng/ml) and IFN-γ (20 ng/ml) for 2 days. M2 macrophages were polarized with: MCSF (10 ng/ml) for 5 days, IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 2 days. Polarized macrophages were stained with antibodies against the stated cell surface molecules and fluorescence was measured by flow cytometry. MFI values a were obtained using FlowJo software version 10.7.1 and histograms b from one representative experiment are shown. Bar graph represents mean ± SD, * p < 0.05, ** p < 0.005 with n = 4 for no apparent CAD, n = 3 for non-obstructive CAD and n = 7 for obstructive CAD

Article Snippet: The adherent monocytes were cultured for 5 days in RPMI 1640 with stable glutamine media supplemented with 10% heat-inactivated fetal bovine serum (Capricorn Scientific, Germany), 1% penicillin-streptomycin (Nacalai Tesque, Japan), and 20 ng/ml recombinant GMCSF (Miltenyi Biotec, Germany) for M1 macrophage or 10 ng/ml recombinant MCSF (Gold Biotechnology, Missouri) to generate M2 macrophages.

Techniques: Marker, Staining, Fluorescence, Flow Cytometry, Software